This reader's dual monochromators for. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. SpectraMax® Microplate Readers and SoftMax® Pro Software. Figure 1. The SpectraMax M2 and M2e are versatile and reliable. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Published Dec 16, 2020 | Updated Dec 16, 2020. keeping with best practices, reader performance should. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Incubation. 0 and 0. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Instrument settings are shown in Figure 3. Softmax Pro GxP Software 6. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. 6 for further experiment, so I'm confused which i would choose for OD value determination. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. SpectraMax® ABS. book Page 7 Wednesday, July 7, 2010 2:09 PM. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. , USA). Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. We use Spectramax M5 microplate reader in our lab. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. . TR-FRET assay. 96-384. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Microbiota DNA was. com A. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Italic and boldface type are used for emphasis. spectramax m5 multi. Molecular Devices SpectraMax® series microplate readers. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. flexibility of monochromator-based systems. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. Western blotting assays were conducted according to previous studies . Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . In brief, RAW 264. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). 306 plate air cal. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 63 1. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. The PBS solution (10. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. edu) to be added as a trained user in iLab. SpectraMax M5 Multi-Mode Microplate Reader. Ltd. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 314 low lamp level warning. 6-384. 3 Version 7. These robust readers have been placed in labs from Antarctica to the. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 1. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Qualify the fluorescence performance of the SpectraMax . SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. However, when a specific probe is not available, or it causes off-target effects on the. 1. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Shop now. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. Offer Ends In: 44 Days 10: 22: 56. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Spectr. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. To block unwanted residual excitation light and minimize background interference, a 570 nm. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The raw data from each individual experiment were normalized to vehicle-treated HK-2. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Selection of Water Constant in PathCheck settings. 9000-0756. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. Western blotting assay. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. SpectraMax® iD5. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 00. 2. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 3. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. Self Service Available. Choose cables that meet the requirement described in this appendix. Access this by going to Help >. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Five preferred modes for diverse bioresearch applications. l. FlexStation™ I. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). Molecular Devices. The cells were incubated with compounds at the indicated doses for 1 h. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. The following document is intended to demonstrate setup of this instrument. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. 3 1. SpectraMax M5e Multi-Mode Microplate Reader. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Wide range of applications. l. 1. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Plasma Tau was determined by high-performance liquid chromatography (Shimadzu ®, model LC 10AD) using a Shimadzu model RF 535 fluorescence detector. The following document is intended to demonstrate setup of this instrument. FlexStation™ III. Microplate reader settings. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). p. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The dual monochromator optics allow the. The dual monochromator optics allow the widest. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Figure 1. Page 28 Chapter 5: Read Modes and Read Types 1. Maximum signal with lowest background. Here the delta mP was 336, and Z’ factor was 0. Add to Cart. Turn on the microplate reader. 00 USD. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Molecular Devices | Life Science Applications | Bioanalytical. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. 312 gain calibration failed. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 1. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. edu) to be added as a trained user in iLab. 315 can't find zero order. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Pick the best speed, sensitivity, and price for your needs. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Microplate Readers are modular and . book Page 7 Wednesday, July 7, 2010 2:09 PM. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. All associated StakMax® stackers. • Tel. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 0 OD for earlier models. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. SpectraMax Glo Steady-Luc Reporter Assay Kit. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. 1step assay with SpectraMax microplate readers. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. D. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Turn on the microplate reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. When a feature or capability applies to only certain readers, this exception is noted. FlexStation® 3 Multi-Mode Microplate Reader. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Learn how to set up protocols, customize reports, export results, and more with this PDF document. 67. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Learn how to set up protocols, customize reports, export results, and more with this PDF document. 7°C (gradient heating design). SpectraMax Microplate Readers. 1 Version 7. Flexible for 6-well to 384-well microplates and cuvettes. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. Anne T. Microplate Readers Biotek Synergy H1 Hybrid Microplate. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Our absorbance plate readers feature our PathCheck Sensor. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. TNF standard curve. 6% for the on-board optics (monochromators). microplate reader. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. ® reader to the computer. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 0 μL) instead of the sample solution was applied as control group, and. The absorbance values at 450 and 570 nm were determined using the SpectraMax M5 microplate reader (Molecular Devices, Silicon Valley, CA, United States). ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Most papers they cultured bacteria to OD600>0. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. e. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 87. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. The assay window spanned four orders of magnitude. AquaMax® 2000. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. #M5) SpectraMax® L Microplate Reader (cat. 6 for further experiment, so I'm confused which i would choose for OD value determination. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. Add to Cart. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. Catalog Number: (89212-396) Supplier: Molecular Devices. Add to Cart. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. SpectraMax® M5e. After being incubated in the dark at 60 ℃ overnight, the supernatants were centrifuged and optical densities were measured at 750, 665, 652 and 470 nm using the SpectraMax M5 microplate reader (Molecular Devices,. 49 2. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. B: Selection of Excitation Wavelength. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. No accessories are needed and SoftMax® Pro Software. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. 1. 1. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. com. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Make sure the microplate is flat against. 0 2. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Self Service Available. Microplate Readers include the M3, M4, M5, and M5e models. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). The spectrophotometric. 2. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). on the SpectraMax Multi-Mode Microplate Readers by issuing a single. 00 USD. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). S. Page 28 Chapter 5: Read Modes and Read Types 1. FlexStation™ II. Our absorbance plate readers feature our PathCheck Sensor technology and. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. Microplate Readers Include the M3, M4, M5, and M5. When a. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Introduction. Bookings are free, but you will need to use iLab to book. 14 Average %CV 0. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 00. es •. The system. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Figure 4: Transcreener ADP. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Molecular Devices. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 6 0. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 1 second. 1** Version 7. The plate reader can measure multiple readouts, including UV and visible absorbance. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 304 signal level saturation. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. 7. SpectraMax® ABS Plus. 5°C at 37°C. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Nontreated cells in DMEM medium were used as a control. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. 0. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Figure 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Fill a clean plate with water. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. Do not touch the clear surfaces of the cuvette. AquaMax® 2000. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. spr. SpectraMax Lmicroplate luminometer. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 05% Tween 20 using a Nunc ELISA plate washer. begin reading microplates with seven mouse clicks. 1–0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Five preferred detection modes. The dual monochromator optics allow. 1. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Qualify the luminescence performance of the SpectraMax . Examples: “Press carefully to engage,” “ Do not. Wide range of applications. Table of Contents. 1 & 7. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Ltd. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. TR-FRET assay principle. Wash Buffer Concentrate was diluted 10-fold. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. 2 only) SpectraMax M-series Microplate readers. 59 6.